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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
23/01/2019 |
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Data da última atualização: |
23/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
PCR-based genotyping of SNP markers in sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 4, p. 651-654, Aug. 2018. |
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DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
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Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
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Thesagro: |
Marcador Molecular; Ovino. |
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Thesaurus Nal: |
Genome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02277naa a2200277 a 4500
001 2104694
005 2019-01-23
008 2018 bl --- 0-- u #d
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 4, p. 651-654, Aug. 2018.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
20/09/2022 |
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Data da última atualização: |
19/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BASSO, M. F.; LOURENCO, I. T.; MOREIRA-PINTO, C. E.; MENDES, R. A. G.; PAES-DE-MELO, B.; NEVES, M. R. das; MACEDO, A. F.; FIGUEIREDO, V.; GRANDIS, A.; MACEDO, L. L. P. de; ARRAES, F. B. M.; COSTA, M. M. do C.; TOGAWA, R. C.; ENRICH-PRAST, A.; MARCELINO-GUIMARÃES, F. C.; GOMES, A. C. M. M.; SILVA, M. C. M. da; FLOH, E. I. S.; BUCKERIDGE, M. S.; ENGLER, J. de A.; SA, M. F. G. de. |
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Afiliação: |
MARCOS FERNANDO BASSO; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CLIDIA EDUARDA MOREIRA-PINTO, Federal University of Brasília; RENEIDA APARECIDA GODINHO MENDES, Federal University of Brasília; BRUNO PAES-DE-MELO; MAYSA ROSA DAS NEVES; AMANDA FERREIRA MACEDO, University of São Paulo; VIVIANE FIGUEIREDO, Federal University of Rio de Janeiro; ADRIANA GRANDIS, University of São Paulo; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; FABRÍCIO BARBOSA MONTEIRO ARRAES; MARCOS MOTA DO CARMO COSTA, Cenargen; ROBERTO COITI TOGAWA, Cenargen; ALEX ENRICH-PRAST, Federal University of Rio de Janeiro; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO; ANA CRISTINA MENESES M GOMES, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ENY IOCHEVET SEGAL FLOH, University of São Paulo; MARCOS SILVEIRA BUCKERIDGE, University of São Paulo; JANICE DE ALMEIDA ENGLER, Université Côte d’Azur, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Planta, v. 256, 83, 2022. |
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Páginas: |
16 p. |
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DOI: |
10.1007/s00425-022-03992-2 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Leonardo Lima Pepino Macedo; Francismar Corrêa Marcelino-Guimaraes; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Palavras-Chave: |
BRS133; Glyma 11G121800; New biotechnology tools; Phytoglobins; PI595099; Plant-nematode interaction; Rootknot nematodes. |
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Thesagro: |
Glycine Max; Nematóide; Soja. |
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Thesaurus NAL: |
Nematoda; Soybeans. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151081/1/Basso-et-al.-2022-GmGlb1.pdf
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Marc: |
LEADER 01644naa a2200529 a 4500
001 2151081
005 2023-01-19
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/s00425-022-03992-2$2DOI
100 1 $aBASSO, M. F.
245 $aOverexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita.$h[electronic resource]
260 $c2022
300 $a16 p.
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Leonardo Lima Pepino Macedo; Francismar Corrêa Marcelino-Guimaraes; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
650 $aNematoda
650 $aSoybeans
650 $aGlycine Max
650 $aNematóide
650 $aSoja
653 $aBRS133
653 $aGlyma 11G121800
653 $aNew biotechnology tools
653 $aPhytoglobins
653 $aPI595099
653 $aPlant-nematode interaction
653 $aRootknot nematodes
700 1 $aLOURENCO, I. T.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMENDES, R. A. G.
700 1 $aPAES-DE-MELO, B.
700 1 $aNEVES, M. R. das
700 1 $aMACEDO, A. F.
700 1 $aFIGUEIREDO, V.
700 1 $aGRANDIS, A.
700 1 $aMACEDO, L. L. P. de
700 1 $aARRAES, F. B. M.
700 1 $aCOSTA, M. M. do C.
700 1 $aTOGAWA, R. C.
700 1 $aENRICH-PRAST, A.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aGOMES, A. C. M. M.
700 1 $aSILVA, M. C. M. da
700 1 $aFLOH, E. I. S.
700 1 $aBUCKERIDGE, M. S.
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 256, 83, 2022.
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Registro original: |
Embrapa Soja (CNPSO) |
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